May 20
John I. Glass,1 Carole Lartigue,1 Vladimir N. Noskov,1 Ray-Yuan Chuang,1Mikkel A. Algire,1 Gwynedd A. Benders,2 Michael G. Montague,1 Li Ma,1 Monzia M. Moodie,1Chuck Merryman,1 Sanjay Vashee,1 Radha Krishnakumar,1 Nacyra Assad-Garcia,1Cynthia Andrews-Pfannkoch,1 Evgeniya A. Denisova,1 Lei Young,1 Zhi-Qing Qi,1Thomas H. Segall-Shapiro,1 Christopher H. Calvey,1 Prashanth P. Parmar,1 Clyde A. Hutchison, III,2Hamilton O. Smith,2 J. Craig Venter1,2,*
2 The J. Craig Venter Institute, 10355 Science Center Drive, San Diego, CA 92121, USA.
Received for publication 9 April 2010. Accepted for publication 13 May 2010.
Source:http://www.sciencemag.org/cgi/content/abstract/science.1190719
Science: Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome
Published Online May 20, 2010 |
RESEARCH ARTICLES
Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome
Daniel G. Gibson,1 We report the design, synthesis, and assembly of the 1.08-Mbp Mycoplasma mycoides JCVI-syn1.0 genome starting from digitized genome sequence information and its transplantation into a Mycoplasma capricolum recipient cell to create new Mycoplasma mycoides cells that are controlled only by the synthetic chromosome. The only DNA in the cells is the designed synthetic DNA sequence, including "watermark" sequences and other designed gene deletionsand polymorphisms, and mutations acquired during the building process. The new cells have expected phenotypic properties and are capable of continuous self-replication.
1 The J. Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850, USA.2 The J. Craig Venter Institute, 10355 Science Center Drive, San Diego, CA 92121, USA.
* To whom correspondence should be addressed. E-mail: jcventer@jcvi.org
Received for publication 9 April 2010. Accepted for publication 13 May 2010.
Source:http://www.sciencemag.org/cgi/content/abstract/science.1190719
